| Seth MacFarlane | Stewie Griffin/Peter Griffin/Brian Griffin/Glen Quagmire/Tom Tucker/Additional Voices |
| Alex Borstein | Lois Griffin/Additional Voices |
| Mila Kunis | Meg Griffin/Additional Voices |
| Seth Green | Chris Griffin/Additional Voices |
| Lori Alan | Diane Simmons |
| Drew Barrymore | Drew Barrymore |
| Noel Blanc | Bugs Bunny/Additional Voices |
| Mike Henry | Cleveland |
| Don LaFontaine | FOX Announcer |
| Will Sasso | Randy Newman |
| Adam West | Mayor Adam West |
| Rene Auberjonois | Odo |
| Jennie Garth | Herself |
| Larry Kenney | Lion-O |
| Lynne Lipton | Cheetara |
| Jason Priestley | Himself |
| Tori Spelling | Herself |
| Michael Clarke Duncan | The Stork |
| Patrick Warburton | Joe Swanson |
